Overview
The E.Z.N.A.® FastFilter Plasmid Maxi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 50-200 mL of bacterial cultures in LB medium typically produces 0.6-1.2 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in vitro transcription, transformation, and other applications.
- Speed – Purification of plasmid DNA < 40="">
- Safety – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High quality – DNA is suitable for a variety of downstream applications
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Downstream Application | Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc. |
| Starting material | 50-200 mL LB culture with OD600 between 2 and 3; or equivalent |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | Manual (centrifugation or vacuum) |
| Throughput | 1 - 24 |
| DNA binding technology | Silica Maxi Spin Column |
| Lysate clearance method | Filtration using Lysate clearance syringe |
| Processing time | <40> |
| Yield | 600-1200 µg for high copy-number; 50-300 µg for low copy-number |
| Special note | Lysate clearance syringe for rapid sample processing |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
D6905 D6924 E.Z.N.A. FastFilter Plasmid DNA Midi & Maxi Kit
SDS
D6924 SDS
SALES SHEET
Product Data
Processing time of plasmid DNA isolation kits
Figure 1. Total time in minutes required for plasmid DNA isolation from pelleting the bacterial cultures to eluting the plasmid using Omega Bio-tek E.Z.N.A.® FastFilter Plasmid Maxi Kit and comparable products from other competitors.
Citations
- Simultaneous nitrogen and carbon removal from swine digester liquor by the Canon process and denitrification Zhaoji Zhang, Yuanyuan Li, Shaohua Chen, Shumei Wang, Xiaodan Bao
