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当前位置: 首页 > 产品中心 > Hybridization_apparatus > Omega Bio-Tek/E.Z.N.A.无内切质粒迷你试剂盒I/免费样品/D6948-00S
商品详细Omega Bio-Tek/E.Z.N.A.无内切质粒迷你试剂盒I/免费样品/D6948-00S
Omega Bio-Tek/E.Z.N.A.无内切质粒迷你试剂盒I/免费样品/D6948-00S
Omega Bio-Tek/E.Z.N.A.无内切质粒迷你试剂盒I/免费样品/D6948-00S
商品编号: D6948-00S
品牌: omegabiotek
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 杂交仪
公司分类: Hybridization_apparatus
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

Plasmid isolated with traditional purification procedures normally contains high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. Up to 5 mL of overnight cultures can be used for high copy number plasmids and 10 mL for low copy number.

The E.Z.N.A.® Endo-Free Plasmid Mini Kit I integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding conditions, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

  • Rapid – 30 minutes or less
  • Safe – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – Endotoxins efficiently reduced (<1 EU/µg)

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Downstream ApplicationSensitive cell line transfection; gene therapy etc.
Starting Material 1-5 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid TypeHigh-copy, low-copy, cosmid DNA
Processing modeManual, centrifugation
Throughput1 - 24
DNA binding technologySilica mini spin column
Lysate clearing methodCentrifugation
Processing Time<30>
Yield15-25 µg for high copy-number; 0.1-5 µg for low copy-number
OtherIncludes endotoxin removal step

Protocol and Resources

Product Documentation & Literature

PROTOCOL

D6948 & D6950 E.Z.N.A. Endo-Free Plasmid DNA Mini I and II Kit

SDS

D6948 SDS

Product Data

Figure 1 Title

Figure 1.  Quality and yield of plasmid DNA purified using the E.Z.N.A.® Endo-Free Plasmid Mini Kit. Plasmid DNA was isolated from 3 mL of 3 different bacterial cultures. DNA was eluted in 100 µL endotoxin-free elution buffer and quantitated using Thermo Fisher’s NanoDrop® 2000 and dynamic turbidity method.

Size

FREE SAMPLE, 5 preps, 50 preps, 200 preps

Format

Miniprep, Endo-free

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等