4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:原厂直采,平行进口,授权代理(蚂蚁淘为您服务)
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > surfactant > Omega Bio-Tek/E.Z.N.A.?无内切质粒Midi试剂盒/免费样品/D6915-00S
商品详细Omega Bio-Tek/E.Z.N.A.?无内切质粒Midi试剂盒/免费样品/D6915-00S
Omega Bio-Tek/E.Z.N.A.?无内切质粒Midi试剂盒/免费样品/D6915-00S
Omega Bio-Tek/E.Z.N.A.?无内切质粒Midi试剂盒/免费样品/D6915-00S
商品编号: D6915-00S
品牌: omegabiotek
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 表面活性剂
公司分类: surfactant
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

The E.Z.N.A.® Endo-Free Plasmid Midi Kit provides an efficient method for the large-scale isolation of low-endotoxin plasmid DNA. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis. Following lysate clearance, endotoxins are removed, and the protocol follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 15-50 mL of bacterial cultures in LB medium typically produces up to 200 µg of high copy number plasmid DNA. Up to 100 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for sensitive downstream applications such as transfection of mammalian cells, cloning, automated fluorescent DNA sequencing (typical reads exceed 800 bp), next-generation sequencing, restriction enzyme digestion, ligation, PCR, in vitro transcription, transformation, etc.

  • Rapid – Purification of plasmid DNA in < 45="">
  • Safe – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – Transfection grade, low-endotoxin plasmid DNA (< 1="">

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Downstream ApplicationTransfection, gene therapy, cloning, in vitro Transcription, Next-GenerationSequencing, PCR, Transformation, restriction digestion, ligation
Starting material15-50 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid typeHigh-copy, low-copy, cosmid DNA
Processing modeManual (centrifugation or vacuum)
Throughput1 - 24
DNA binding technologySilica Midi Spin Column
Lysate clearance methodFiltration using Lysate clearance syringe
Processing time< 45="">
Yield100-250 µg for high copy-number; 10-50 µg for low copy-number
Special noteIncludes endotoxin removal step

Protocol and Resources

Product Documentation & Literature

PROTOCOL

D6915 D6926 Endo-Free Plasmid Midi Maxi Kit

SDS

D6915 SDS

SALES SHEET

Product Data

Table 1.  pEGFP-N1 plasmid was purified from 50 mL DH5α cultures harboring the plasmid using Omega Bio-tek’s E.Z.N.A.® Endo-Free Plasmid Midi Kit following manufacturer’s recommended protocol. Plasmid DNA was eluted in 500 µL volume and quantified using Thermo Scientific’s NanoDrop™ 2000c system. The Endotoxin concentration was estimated based on a Limulus Amoebocyte Lysate (LAL) assay.

Size

FREE SAMPLE, 2 preps, 10 preps, 25 preps, 100 preps

Format

Midiprep, Endo-free

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等