Overview
The E.Z.N.A.® MicroElute RNA Clean Up Kit is designed to purify and concentrate RNA (from 50 µg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following the phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications requiring highly concentrated RNA samples.
- Recovery of RNA from enzymatic reactions in 15 minutes
- No organic extractions
- Clean up RNA isolated by others methods (e.g. crude preps from organic extraction methods)
- Concentrates small amounts of RNA to 10-15 µL
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Downstream application | Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 10-15 µL |
| Starting material | Enzymatic reaction |
| Starting amount | Up to 10 µg RNA |
| DNA recovered | >90% recovery, RNA > 200 bases |
| Processing mode | Manual, centrifugation |
| Throughput | 1 |
| DNA binding technology | Silica MicroElute spin column |
| Binding capacity | 10 μg |
| Processing time | <15> |
| Special note | Low elution volume, suitable for RNA desalting too |
Kit Components
| Item | Available Separately |
|---|---|
| MicroElute LE RNA Mini Columns | View Product |
| 2 mL Collection Tubes | View Product |
| QVL Lysis Buffer | View Product |
| RNA Wash Buffer II | View Product |
| DEPC Water | View Product |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
R6247 E.Z.N.A. MicroElute RNA Cleanup Kit Combo
SDS
R6247 SDS
Product Data
RNA was intact after being purified using the MicroElute RNA Clean Up Kit
Figure 1. Total RNA purified using the MicroElute RNA Clean Up Kit. Total RNA was purified from 15 µL RNA original solution and eluted in 15 µL elution buffer. Total RNA (2 µL) was analyzed on a 1% agarose gel. Lanes 1-4: after purification, C: before purification.
Citations
- Hoffman, Alyson M., et al. “Heterogeneous Translational Landscape of the Endoplasmic Reticulum Revealed by Ribosome Proximity Labeling and Transcriptome Analysis.” Journal of Biological Chemistry, vol. 294, no. 22, 31 May 2019, pp. 8942–8958, www.jbc.org/content/294/22/8942.short, 10.1074/jbc.RA119.007996. Accessed 1 June 2020.
