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当前位置: 首页 > 产品中心 > Anti_Swine > Omega Bio-Tek/Mag-Bind® TotalPure NGS/500-ml/M1378-02
商品详细Omega Bio-Tek/Mag-Bind® TotalPure NGS/500-ml/M1378-02
Omega Bio-Tek/Mag-Bind® TotalPure NGS/500-ml/M1378-02
Omega Bio-Tek/Mag-Bind® TotalPure NGS/500-ml/M1378-02
商品编号: M1378-02
品牌: omegabiotek
市场价: ¥71094.00
美元价: 35547.00
产地: 美国(厂家直采)
公司:
产品分类: 抗猪
公司分类: Anti_Swine
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent-to-sample ratio used, giving the user flexibility to perform left, right or double-sided size selection. This product is designed for both manual and fully automated purification of DNA and RNA samples, as well as for the purification of PCR products. The system combines Omega Bio-tek’s proprietary chemistries with reversible nucleic acid-binding properties of magnetic beads to selectively bind fragments and eliminate excess nucleotides, primers, and small, non-targeted products such as primer-dimers. Purified DNA and RNA is suitable for a variety of downstream applications such as NGS library preparation, microarrays, automated fluorescent sequencing and restriction enzyme digestion.

  • No protocol change against major competitor
  • Double-sided size selection
  • DNA Clean-Up: PCR Clean-Up
  • RNA Clean-Up: cDNA or RNA purification
  • Manual or adaptable to most open-ended liquid handlers
  • Significant cost savings
  • 96- or 384-well formats

Protocols are available for the following automated platforms:

  • Hamilton Microlab® STAR
  • Hamilton Microlab® NIMBUS
  • KingFisher™, BioSprint®, and MagMAX® 96

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Downstream applicationCloning, NGS, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume15 µL or above
Starting materialDNA or RNA: PCR products, gDNA, cDNA
Starting amountScalable
DNA recovered>90% recovery for DNA >100 bp
Processing modeAutomated; manual
Throughput96-384 samples per run
DNA binding technologyMagnetic beads
Storage2°C - 8°C
Special noteSize selection by varying beads ratio

Protocol and Resources

Product Documentation & Literature

PROTOCOL

M1378 Mag-Bind TotalPure NGS

QUICK GUIDE

M1378 Mag-Bind TotalPure NGS

SDS

M1378 SDS

SALES SHEET

REFERENCE

University of Oregon Evaluation of Mag-Bind TotalPure NGS for DNA Size Selection

APPLICATION NOTE

Automated DNA Cleanup for PCR and NGS Workflows: Mag-Bind® TotalPure NGS on Tecan Fluent® 780 Workstation

Product Data

Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent double-side size selection.

Figure 1.  Electropherogram overlay of the double-sided size selection on sheared gDNA at 0.8x/0.7x ratio set using Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable kit from Company A following manufacturer’s recommended protocols. The DNA was eluted in 25 µL and analyzed on Agilent’s TapeStation® 2200.

Mag-Bind TotalPure NGS from Omega Bio-tek has excellent recovery of targeted DNA fragments.

Figure 2.  10 µL of 50 bp ladder was purified with Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable product from Company A following manufacturer’s recommended protocols. The DNA was eluted in 20 µL and analyzed on Agilent’s TapeStation® 2200.

Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent RNA clean-up in a wide concentration range.

Figure 3.  10 µL of RNA at 50 ng/µL and 5 ng/µL was cleaned up with Omega Bio-tek’s Mag-Bind® TotalPure NGS following manufacturer’s recommended protocols. The RNA was eluted in 20 µL and analyzed on Agilent’s TapeStation® 2200. Recovery rates were 85-92% respectively.

Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent double-sided size selection on DNA from NGS library prep.

Figure 4.  Next-generation sequencing libraries prepared from 350 ng sheared genomic DNA using Kapa Biosystem’s HyperPrep Kits (KK8504) and Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable product from Company A on the Hamilton Microlab® STAR™. Mag-Bind® TotalPure NGS was used for 2 clean up step (0.8x and 1.0x) following Kapa Biosystems’ recommended protocol for clean up. DNA was analyzed on Agilent’s TapeStation® 2200 following library construction.

Citations

View Citations
  • Bao, Hong, et al. “Construction of a DNA Vaccine Based on the Mycobacterium Tuberculosis Ag85A/MPT64 Fusion Gene and Evaluation of Its Immunogenicity.” Molecular Medicine Reports, vol. 6, no. 6, 30 Nov. 2012, pp. 1375–1378, www.spandidos-publications.com/mmr/6/6/1375?text=fulltext, 10.3892/mmr.2012.1109. Accessed 1 June 2020.
  • Chu, Xin, et al. “Characterization of UDP-Glucose Dehydrogenase from Pasteurella Multocida CVCC 408 and Its Application in Hyaluronic Acid Biosynthesis.” Enzyme and Microbial Technology, vol. 85, 1 Apr. 2016, pp. 64–70, www.sciencedirect.com/science/article/pii/S0141022915300946?casa_token=RbXXff7WCJMAAAAA:AR_jG3Ox_yViS0rAsOqanFCcDgEkO5sZQF6bDxTxCpJzOpkYBM9LKiqayq8VG_7zTfFgGN78hc4, 10.1016/j.enzmictec.2015.12.009. Accessed 1 June 2020.
  • Dong, Xingyu, et al. “Downregulation of HTATIP2 Expression Is Associated with Promoter Methylation and Poor Prognosis in Glioma.” Experimental and Molecular Pathology, vol. 98, no. 2, 1 Apr. 2015, pp. 192–199, www.sciencedirect.com/science/article/pii/S0014480015000155?casa_token=CIVGtzRE8-sAAAAA:uAqLbIzZkMrpl49dlRDOp3mTtaZMsyZ-44UEUSEXdKCfVuuWpBWJRSIZ2aW0FKl3slalsPfcs00, 10.1016/j.yexmp.2015.01.013. Accessed 1 June 2020.
  • Gao, Xue, et al. “Genomic Study of Polyhydroxyalkanoates Producing Aeromonas Hydrophila 4AK4.” Applied Microbiology and Biotechnology, vol. 97, no. 20, 3 Sept. 2013, pp. 9099–9109, 10.1007/s00253-013-5189-y. Accessed 1 June 2020.
  • Hao, Haibang, et al. “Complete mitochondrial genome of a new vole Proedromys liangshanensis (Rodentia: Cricetidae) and phylogenetic analysis with related species: Are there implications for the validity of the genus Proedromys?.” Mitochondrial DNA 22.1-2 (2011): 28-34.
  • Huang, Qichao, et al. “RNA-Seq Analyses Generate Comprehensive Transcriptomic Landscape and Reveal Complex Transcript Patterns in Hepatocellular Carcinoma.” PLoS ONE, vol. 6, no. 10, 17 Oct. 2011, www.ncbi.nlm.nih.gov/pmc/articles/PMC3197143/, 10.1371/journal.pone.0026168. Accessed 1 June 2020.
  • Jiang, Han-Peng, et al. “Determination of Formylated DNA and RNA by Chemical Labeling Combined with Mass Spectrometry Analysis.” Analytica Chimica Acta, vol. 981, 15 Aug. 2017, pp. 1–10, www.sciencedirect.com/science/article/pii/S0003267017307122?casa_token=xt2DarDWL_QAAAAA:VNeMkTQYF8rqKrXCSIozXzlMQPcKL7OifWYX3AXJvn7yDqT_jGdgwyuNbs1IEJTr5l0d2iWobNs, 10.1016/j.aca.2017.06.009. Accessed 1 June 2020.
  • Li, Haiyu, et al. “Bmi-1 Regulates Epithelial-to-Mesenchymal Transition to Promote Migration and Invasion of Breast Cancer Cells.” International Journal of Clinical and Experimental Pathology, vol. 7, no. 6, 15 May 2014, pp. 3057–3064, www.ncbi.nlm.nih.gov/pmc/articles/PMC4097277/. Accessed 1 June 2020.
  • Li, J.M., et al. “Genetic Mechanism Associated with Congenital Cytomegalovirus Infection and Analysis of Effects of the Infection on Pregnancy Outcome.” Genetics and Molecular Research, vol. 14, no. 4, 2015, pp. 13247–13257, www.geneticsmr.com/year2015/vol14-4/pdf/gmr6714.pdf, 10.4238/2015.october.26.21. Accessed 1 June 2020.
  • Liu, Feiwei, et al. “Taxonomic status of Tetraophasis obscurus and Tetraophasis szechenyii (Aves: Galliformes: Phasianidae) based on the complete mitochondrial genome.” Zoological science 31.3 (2014): 160-167.
  • Li, Yuan-Yuan, et al. “Protocols for Establishing Fungi-Protocorm Culture.” Springer Protocols Handbooks, 2018, pp. 61–69, 10.1007/978-1-4939-7771-0_3. Accessed 1 June 2020.
  • Noor‐Mohammadi, Samaneh, Azadeh Pourmir, and Tyler W. Johannes. “Method to assemble and integrate biochemical pathways into the chloroplast genome of Chlamydomonas reinhardtii.” Biotechnology and bioengineering 109.11 (2012): 2896-2903.
  • Pimentel, Tânia, et al. “Bacterial Communities 16S RDNA Fingerprinting as a Potential Tracing Tool for Cultured Seabass Dicentrarchus Labrax.” Scientific Reports, vol. 7, no. 1, 19 Sept. 2017, pp. 1–10, www.nature.com/articles/s41598-017-11552-y, 10.1038/s41598-017-11552-y. Accessed 1 June 2020.
  • Yue, Hao, et al. “Two Novel Mitogenomes of Dipodidae Species and Phylogeny of Rodentia Inferred from the Complete Mitogenomes.” Biochemical Systematics and Ecology, vol. 60, 1 June 2015, pp. 123–130, www.sciencedirect.com/science/article/pii/S0305197815000897?casa_token=8xflC0yV3lEAAAAA:QJ6c9zEmHvBRh6JCVbY7V47tkiOVYdJhi9NCUrRaYYF7uQwIkBqrDeUIJhgXtLWPmAbJ8-EFk6Y, 10.1016/j.bse.2015.04.013. Accessed 1 June 2020.
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Format

Magnetic beads

Size

FREE SAMPLE, 5 mL, 50 mL, 500 mL

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等