Overview
The E.Z.N.A.® Bacterial RNA Kit is designed for isolation of high quality total RNA from variety of bacterial strains. Up to 1 x 109 log- phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridization techniques.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Downstream Application | PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
| Elution Volume | 50-100 μL |
| Starting Material | Bacteria |
| Starting Amount | Up to 3 mL culture, 1 x 109 cells |
| RNA Yield | up to 100 µg |
| Processing Mode | Manual, centrifugation/vacuum |
| Throughput | 1 - 24 |
| RNA Binding Technology | Silica Mini spin column |
| Binding Capacity | 100 µg |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
R6950 E.Z.N.A. Bacterial RNA Kit
SDS
R6950 SDS
Product Data
Total RNA purified using E.Z.N.A.® Bacterial RNA Kit.
Figure 1. Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from 2 mL bacterial samples as follows: sample 1 from Pseudomonas arginosa, sample 2 from Enterococcus faecalis, and sample 3 from Staphylococcus aureus. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.
Total RNA purified using E.Z.N.A.® Bacterial RNA Kit.
Figure 2. Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from lag phase E. coli cultures as follows: sample 1 from 0.5 mL culture, sample 2 from 1.0 mL culture, sample 3 from 2 mL culture, and sample 4 from 3 mL culture. Lanes 1-4 represent the first 100 µL elution. Total RNA (50% of total purified RNA) was analyzed on a denaturing formaldehyde agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.
Publications
- Molecular cloning and characterization of bile salt hydrolase in Lactobacillus casei zhang Wen Yi Zhang, Ri Na Wu, Zhi Hong Sun, Tian Song Sun, He Meng, He Ping Zhang
- Triclosan resistance in clinical isolates of Acinetobacter baumannii Yagang Chen, Borui Pi, Hua Zhou, Yunsong Yu, Lanjuan Li
