Overview
The E.Z.N.A.® Viral RNA Kit is designed for the isolation of viral DNA and viral RNA from cell-free fluids such as plasma, serum, urine and cell culture supernatant. The procedure completely removes contaminants and enzyme inhibitors making viral RNA isolation fast, convenient and reliable. The kit is also suitable for the isolation of total RNA from cultured cells, tissues and bacteria. RNA purified using the E.Z.N.A.® Viral RNA method is ready for all downstream applications such as RT-PCR.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Starting Amount | 150 µL |
| Starting Material | Cell free fluids such as plasma, serum, urine and cell culture supernatant |
| Elution Volume | 20-50 μL |
| Technology | HiBind® RNA Mini Column |
| Processing Mode | Manual, Centrifugation/Vacuum |
| Throughput | 1-24 |
Kit Components
| Item | Available Separately |
|---|---|
| HiBind® RNA Mini Columns | View Product |
| 2 mL Collection Tubes | View Product |
| QVL Lysis Buffer | View Product |
| RNA Wash Buffer II | View Product |
| VHB Buffer | View Product |
| Carrier RNA (Poly A) | --- |
| Nuclease-Free water | View Product |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
R6874 E.Z.N.A.® Viral RNA Kit
PROTOCOL
(R6874) Supplementary Protocol for NP Swabs, Aspirates and BAL Samples
QUICK GUIDE
R6874 E.Z.N.A.® Viral RNA Kit Quick Guide
SDS
R6874 SDS
SALES SHEET
Product Data
Viral RNA extracted using E.Z.N.A.® Viral RNA Kit was PCR inhibitor free
Figure 1. 25 µL of Zeptomatrix Influenza A/B Positive Control was spiked into 125 µL serum samples and then Viral RNA was extracted using the E.Z.N.A.® Viral RNA Kit . 2 µL of Eluted RNA was used as a template in a 20 µL SYBR® qPCR reaction. C: inhibitor-free control; T: RNA sample extracted with kit. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template RNA in free of inhibitors.
Publications
- “Production of Quantum Dots-Containing Influenza Virus …” Bio-Protocol.Org, bio-protocol.org/e3366. Accessed 14 Mar. 2020.
- Qin, Chong, et al. “Production of Quantum Dots-Containing Influenza Virus Particles for Studying Viral Uncoating Processes.” BIO-PROTOCOL, vol. 9, no. 18, 2019, doi:10.21769/bioprotoc.3366.
- Li, Qiuchen, et al. “A New Strategy for the Detection of Chicken Infectious Anemia Virus Contamination in Attenuated Live Vaccine by Droplet Digital PCR.” BioMed Research International, 2019, www.hindawi.com/journals/bmri/2019/2750472/.
- Deng, Shaofeng, et al. “Host Cell Protein PSMB10 Interacts with Viral NS3 Protein and Inhibits the Growth of Classical Swine Fever Virus.” Virology, vol. 537, Nov. 2019, pp. 74–83, doi:10.1016/j.virol.2019.05.017.
- Joglekar, Alok V., et al. T Cell Receptor Immunotherapy Drives Human Immunodeficiency Virus Evolution in Humanized Mice. Mar. 2019, doi:10.1101/574608.
- Yuan, Jin, Mengjiao Zhu, et al. “Classical Swine Fever Virus Induces Pyroptosis in the Peripheral Lymphoid Organs of Infected Pigs.” Virus Research, vol. 250, May 2018, pp. 37–42, doi:10.1016/j.virusres.2018.04.004.
- Yuan, Jin, Zhiyong Han, et al. “Atypical Porcine Pestivirus as a Novel Type of Pestivirus in Pigs in China.” Frontiers in Microbiology, vol. 8, May 2017, doi:10.3389/fmicb.2017.00862.
- Qin, Chong, et al. “Production of Quantum Dots-Containing Influenza Virus Particles for Studying Viral Uncoating Processes.” BIO-PROTOCOL, vol. 9, no. 18, 2019, doi:10.21769/bioprotoc.3366.
