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当前位置: 首页 > 产品中心 > Transfection > Omega Bio-Tek/E-Z 96®植物DNA DS试剂盒/免费样品/D1411-00S
商品详细Omega Bio-Tek/E-Z 96®植物DNA DS试剂盒/免费样品/D1411-00S
Omega Bio-Tek/E-Z 96®植物DNA DS试剂盒/免费样品/D1411-00S
Omega Bio-Tek/E-Z 96®植物DNA DS试剂盒/免费样品/D1411-00S
商品编号: D1411-00S
品牌: omegabiotek
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 转染试剂
公司分类: Transfection
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

The E-Z 96® Plant DNA DS Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications.

This procedure relies on the well-established properties of the cationic detergent, cetyltrimethylammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB, binding conditions are adjusted, and DNA is purified using an E-Z 96® DNA Plate. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Starting Amount50 mg
Starting MaterialFresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content.
Elution Volume150 µL
TechnologySilica 96-well plate
Processing ModeManual
Throughput96

Protocol and Resources

Product Documentation & Literature

PROTOCOL

D1411 E-Z 96® Plant DNA DS Kit

SDS

D1411 SDS

SALES SHEET

APPLICATION NOTE

Rapid, High Performance and Cost-Effective Plant DNA Extractions

Product Data

DNA yields from various plant types are significantly higher using E-Z 96® Plant DNA DS Kits than using a competing product.

Figure 1. Comparison of DNA yield from multiple crops. 40-50 mg of respective fresh leaf tissue was extracted in triplicate according to manufacturer’s recommended protocols and eluted in 100 µL. DNA analyzed with fluorescent DNA-based quantification method. Total yield was divided by total tissue amount to show ng of DNA per mg of leaf tissue.

Size

FREE SAMPLE, 1 x 96 Preps, 4 x 96 Preps

Format

96-well filter plate

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等