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当前位置: 首页 > 产品中心 > CRISPR > Omega Bio-Tek/E.Z.N.A.?通用病原体试剂盒/免费样品/D4035-00S
商品详细Omega Bio-Tek/E.Z.N.A.?通用病原体试剂盒/免费样品/D4035-00S
Omega Bio-Tek/E.Z.N.A.?通用病原体试剂盒/免费样品/D4035-00S
Omega Bio-Tek/E.Z.N.A.?通用病原体试剂盒/免费样品/D4035-00S
商品编号: D4035-00S
品牌: omegabiotek
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: CRISPR基因敲除
公司分类: CRISPR
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

The E.Z.N.A.® Universal Pathogen Kit allows for the rapid and reliable isolation of high-quality host genomic DNA, gram-positive and negative bacterial DNA, fungal spore DNA and viral DNA and viral RNA from tissue, urine, serum, and fecal samples. Elution volumes as low as 15 µL can be used to maintain higher nucleic acid concentrations.

The system combines Omega Bio-tek’s MicroElute columns with RBB Buffer to eliminate PCR inhibiting compounds within the samples and elute highly concentrated DNA. Purified DNA is suitable for PCR, restriction digestion and hybridization applications. There are no organic extractions, thus reducing plastic waste and hands-on time. Multiple samples can be processed in parallel.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Starting Amount 25-30 mg tissue, 250 µL fecal sample / serum / urine/blood,
Starting Material tissue, urine, serum, and fecal sample
Elution Volume15-100 μL
TechnologySilica MicroElute spin column
Processing ModeManual, Centrifugation/Vacuum
NoteHost genomic DNA, gram positive and negative bacterial DNA, fungal spore DNA, and viral DNA and RNA

Kit Components

ItemAvailable Separately
Disruptor TubesView Product
MicroElute® LE DNA ColumnsView Product
2 mL Collection Tubes /product/2-0ml-tube-cap-less-graduated-100-pk-50pk-cs/">View Product
SLX-Mlus BufferView Product
DS BufferCall for Pricing
PCP Buffer---
RBB BufferView Product
HBC BufferView Product
DNA Wash BufferView Product
Elution BufferView Product
Proteinase K SolutionView Product

Protocol and Resources

Product Documentation & Literature

PROTOCOL

D4035 E.Z.N.A.® Universal Pathogen Kit

SDS

D4035 SDS

SALES SHEET

Product Data

Using E.Z.N.A.® Universal Pathogen Kit on various samples produced better quality DNA.

Figure 1. Human fecal samples suspended in PBS solution were spiked into corresponding organisms. Fecal samples were then processed according to each manufacturer’s recommended protocols. qPCR was performed in triplicate for each sample using primers specific for the target organism. Data shown are averages on triplicate reactions.

Citations

View Citations
  • Lin, Qiuyuan, et al. “Real-Time Fluorescence Loop-Mediated Isothermal Amplification Assay for Rapid and Sensitive Detection of Streptococcus Gallolyticus Subsp. Gallolyticus Associated with Colorectal Cancer.” Analytical and Bioanalytical Chemistry, vol. 411, no. 26, 6 Aug. 2019, pp. 6877–6887, 10.1007/s00216-019-02059-8. Accessed 1 June 2020.

Size

FREE SAMPLE, 5 preps, 50 preps

Format

Miniprep

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等