4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:原厂直采,平行进口,授权代理(蚂蚁淘为您服务)
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > RNA_interference > Omega Bio-Tek/Mag-Bind®环境DNA 96试剂盒/免费样品/M5645-00S
商品详细Omega Bio-Tek/Mag-Bind®环境DNA 96试剂盒/免费样品/M5645-00S
Omega Bio-Tek/Mag-Bind®环境DNA 96试剂盒/免费样品/M5645-00S
Omega Bio-Tek/Mag-Bind®环境DNA 96试剂盒/免费样品/M5645-00S
商品编号: M5645-00S
品牌: omegabiotek
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: RNA干扰
公司分类: RNA_interference
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

The Mag-Bind® Environmental DNA 96 Kit allows rapid and reliable isolation of high-quality DNA from soil and water samples. The Mag-Bind® Environmental DNA 96 Kit can isolate microbial DNA from yeast, fungi, and gram-positive or negative bacteria. Up to 96 one hundred mg soil samples can be processed in 120 minutes using automated liquid handlers or magnetic processors. Omega Bio-tek’s unique cHTR Reagent effectively removes humic acid and other PCR inhibitors allowing for purified DNA to be suitable for PCR, 16S sequencing, Whole Genome Sequencing, and Next-Generation Sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.

  • Ceramic Beads pre-aliquoted in a convenient 96-well format
  • Unique inhibitor removal Reagent
  • Automation Friendly
Protocols are available for the following automated platforms:

  • Hamilton Microlab® STAR
  • Hamilton Microlab® NIMBUS
  • KingFisher™, BioSprint®, and MagMAX® 96

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Downstream ApplicationqPCR, PCR, Next Generation Sequencing
Starting materialUp to 250 mg soil or 1 water filter
Target Nucleic AcidGram Negative and positive bacteria, yeast, fungal
Processing modeAutomated or Manual
Throughput96 samples
Processing time120 minutes
DNA binding technologyMagnetic Beads
YieldDependent on sample biomass

Kit Components

ItemAvailable Separately
E-Z 96 Disruptor Plate C PlusView Product
96-well Racked Microtubes---
Caps for Racked Microtubes---
SLX-Mlus BufferView Product
DS BufferCall for Pricing
P2 BufferView Product
XP1 Buffer---
VHB BufferView Product
SPM Wash BufferView Product
Elution BufferView Product
Mag-Bind® Particles RQCall for Pricing
cHTR Reagent---
RNase AView Product

Protocol and Resources

Product Documentation & Literature

PROTOCOL

M5645 Mag-Bind® Environmental DNA 96 Kit

SDS

M5645 SDS

APPLICATION NOTE

Magnetic bead-based solution for DNA extraction from environmental samples using Mag-Bind® Environmental DNA 96 Kit from Omega Bio-tek

Product Data

DNA from soil samples using Mag-Bind® Environmental Kit had higher yield and quality, less inhibitor than using a leading competitor’s product

Figure 1.  Outdoor soil (250mg) was spiked with an approximately 1mL culture of Bacillus subtilis and DNA was extracted using Mag-Bind® Environmental kit (Omega Bio-tek) and Soil DNA Isolation kit (Company Q) following manufacturer’s recommended protocols. The isolations were carried out in quadruplicate for the Omega kit and in duplicate for the competitor’s kit. DNA was quantified using Thermo Scientific’s NanoDrop® 2000c. The quality of the purified DNA was analyzed by performing real-time PCR using Bacillus subtilis specific primers on undiluted and 10-fold diluted DNA isolated from soil extraction protocol.

qPCR Comparison From Water Samples

Figure 2.  Pond water (100mL) was spiked with approximately 1mL culture each of B. subtilis and E. coli harboring pGEM plasmid and was filtered through Sterivex (Millipore SVGPL10RC) filters. Upon filtration, DNA was extracted from these filters in quadruplicate following the manufacturer’s recommended protocols. DNA was quantified using Thermo Scientific’s NanoDrop® 2000c. The quality of the purified DNA was analyzed by performing real-time PCR using Bacillus subtilis specific primers on 10-fold diluted and 100-fold diluted DNA isolated from water extraction protocol.

Size

FREE SAMPLE, 1 x 96 Preps, 4 x 96 Preps

Format

Magnetic beads

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等