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当前位置: 首页 > 产品中心 > Genetic_mutations > Omega Bio-Tek/E.Z.N.A.?植物DNA DS试剂盒/5-preps/D2411-00
商品详细Omega Bio-Tek/E.Z.N.A.?植物DNA DS试剂盒/5-preps/D2411-00
Omega Bio-Tek/E.Z.N.A.?植物DNA DS试剂盒/5-preps/D2411-00
Omega Bio-Tek/E.Z.N.A.?植物DNA DS试剂盒/5-preps/D2411-00
商品编号: D2411-00
品牌: omegabiotek
市场价: ¥388.00
美元价: 194.00
产地: 美国(厂家直采)
公司:
产品分类: 基因突变
公司分类: Genetic_mutations
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications.

This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and binding conditions are adjusted and DNA is purified using a HiBind® DNA Mini Columns. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.

  • Rapid – Homogenizer Columns allow for faster processing
  • Versatile – Reliable results from a variety of sample types
  • Safe – No organic extractions
  • High-quality – Purified DNA suitable for most applications

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Starting AmountUp to 50 mg wet tissue
Starting MaterialFresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content
Elution Volume50-100 μL
TechnologyHiBind® DNA Mini Column
Processing ModeManual
Throughput1-24
NoteCTAB lysis

Kit Components

ItemAvailable Separately
HiBind® DNA Mini ColumnsView Product
Homogenizer Mini ColumnsView Product
2 mL Collection TubesView Product
CSPL BufferView Product
Proteinase K SolutionView Product
RBB BufferView Product
XP2 BufferView Product
HBC BufferView Product
DNA Wash BufferView Product
Elution BufferView Product

Protocol and Resources

Product Documentation & Literature

PROTOCOL

D2411 E.Z.N.A.® Plant DNA DS Kit

SDS

D2411 SDS

SALES SHEET

APPLICATION NOTE

Rapid, High Performance and Cost-Effective Plant DNA Extractions

Product Data

DNA yields from various plant types are significantly hgiher using E.Z.N.A.® Plant DNA DS Kit than using a competing product.

Figure 1. Comparison of DNA yield from multiple crops. 40-50 mg of respective fresh leaf tissue was extracted in triplicate according to manufacturer’s recommended protocols and eluted in 100 µL. DNA analyzed with fluorescent DNA-based quantification method. Total yield was divided by total tissue amount to show ng of DNA per mg of leaf tissue.

DNA purified using E.Z.N.A.® Plant DNA DS Kit has less PCR inhibitors than using a competing product.

Figure 2. qPCR comparison from corn samples. Real-time PCR with corn-specific primers was performed on triplicates of undiluted, 10-fold and 100-fold dilutions of DNA. DNA was isolated using Omega Bio-tek’s E.Z.N.A.® Plant DNA DS Kit and a comparable column-based kit from Company Q. Omega Bio-tek’s kit not only has significantly higher yields but also less qPCR inhibition when compared to that of Company Q’s.

DNA Yield and Quality from Potato Leaf and Corn Leaf Powder

Figure 3. Genomic DNA was purified from either 50 mg potato leaf or 30 mg corn leaf powder with the E.Z.N.A. Plant DNA DS Kit. DNA concentration determined by optical density measurements with NanoDrop® 2000c. Total elution volume was 100 µL.

High Molecular Weight Genomic DNA

Figure 4. Genomic DNA was purified from 50 mg potato leaf with the E.Z.N.A. Plant DNA DS Kit.  5 µL eluate DNA was analyzed on a 1% Agarose gel.

DNA extracted using E.Z.N.A.® Plant DNA DS Kit is inhibitor free

Figure 5. Genomic DNA was extracted from 50 mg potato leaf and 30 mg corn lead powder using the E.Z.N.A. Plant DNA DS Kit . 2 µL of Eluted DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. C: inhibitor-free control; T: gDNA samples. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors

Publications

View Publications
  • Duccio Migliorini, Luisa Ghelardini, Elena Tondini, Nicola Luchi, Alberto Santini The potential of symptomless potted plants for carrying invasive soilborne plant pathogens
  • Paulo Gouveia, Margarida Teixeira Santos, José Eduardo Eiras-Dias, Gustavo Nolasco Five phylogenetic groups identified in the coat protein gene of grapevine leafroll-associated virus 3 obtained from Portuguese grapevine varities
Format

Miniprep

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等