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当前位置: 首页 > 产品中心 > Transfection > Omega Bio-Tek/Mag-Bind®cfDNA试剂盒/200 preps/M3298-02
商品详细Omega Bio-Tek/Mag-Bind®cfDNA试剂盒/200 preps/M3298-02
Omega Bio-Tek/Mag-Bind®cfDNA试剂盒/200 preps/M3298-02
Omega Bio-Tek/Mag-Bind®cfDNA试剂盒/200 preps/M3298-02
商品编号: M3298-02
品牌: omegabiotek
市场价: ¥41820.00
美元价: 20910.00
产地: 美国(厂家直采)
公司:
产品分类: 转染试剂
公司分类: Transfection
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

The Mag-Bind® cfDNA Kit is designed for rapid and reliable isolation of circulating DNA from 500-8,000 µL plasma or serum samples. The Mag-Bind® cfDNA Kit can be processed manually or on an automated platform. The procedure eliminates the need for funnels and vacuum steps, providing hands-free operation in automated protocols.

The uniquely formulated binding buffer allows for large sample volumes to be processed in automated formats which allow for 4 mL of serum or plasma to be processed in a single well in 24-well plate format. The magnetic response of the Mag-Bind® Particles CH allows for fast magnetization during steps requiring high volumes, and the high binding capacity allows for reduced amounts of magnetic particles required, thus reducing the elution volume down to 50 µL for 4mL serum or plasma samples.

The Mag-Bind ® cfDNA Kit has also been automated to process 8 mL samples on Hamilton platforms. Please contact us for automation support.

Protocols are available for the following automated platforms:

  • Hamilton Microlab® STAR
  • Hamilton Microlab® NIMBUS
  • KingFisher™, BioSprint®, and MagMAX® 96

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Sample TypePlasma/Serum
Sample Volume500-8000 µL
TechnologyMagnetic Beads
ProcessingManual or Automated
AutomatableYes
Processing Time2 hours for 96 (1mL) samples
FormatTube, 24-well, 96-well
Elution Volume50 µL

Kit Components

ItemAvailable Separately
Mag-Bind® Particles CHCall for Pricing
DS BufferCall for Pricing
JSB BufferView Product
GT7 Buffer v1.1Call for Pricing
SPW BufferView Product
Elution BufferView Product
Proteinase K SolutionView Product

Protocol and Resources

Resources & Literature

PROTOCOL

M3298 Mag-Bind® cfDNA Kit

SDS

M3298 SDS

SALES SHEET

APPLICATION NOTE

Extraction & Quality Analysis of Circulating, Cell-Free DNA from Serum Samples Using Omega Bio-tek Kits

APPLICATION NOTE

Automated Circulating Cell-Free DNA Extraction from 8 mL Sample Volumes

APPLICATION NOTE

Strategies for retrieving cfDNA of short fragment lengths using Mag-Bind® cfDNA kit (M3298) from Omega Bio-tek

Product Data

Cell-free DNA purified using Omega Bio-tek’s Mag-Bind ® cfDNA Kit has little genomic DNA carryover

Figure 1. Electropherogram overlay of purified DNA from 4 mL serum. 4 mL of unspiked serum was purified using kits from Omega Bio-tek and Company Q following manufacturer’s recommended protocols. Purified DNA was analyzed on Agilent’s TapeStation® 2200.

High quality and yield for cell-free DNA purified using Omega Bio-tek’s Mag-Bind ® cfDNA Kit Mag-Bind® cfDNA kit

Figure 2.  Electropherogram overlay of purified DNA from 1 mL serum. Sheared, bacterial DNA was spiked into serum samples and isolated using Omega Bio-tek’s Mag-Bind cfDNA Kit and a column-based kit from Company Q. Real-time PCR was performed using 16s bacteria-specific primers at 1X and 10X dilutions. Purified DNA was analyzed on Agilent’s TapeStation® 2200. The Omega Bio-tek kit was able to capture the circulating, cell-free DNA with no genomic DNA contamination. In contrast, Company Q’s eluate contained high molecular weight fragments indicating the presence of genomic DNA in the circulating DNA isolation.

cfDNA purified using Omega Bio-tek’s cfDNA kit contains no PCR inhibitors

Table 1. Real-time PCR with 16S bacterial-specific primers was performed on triplicates of undiluted and 10-fold dilutions of DNA isolated from samples purified [figure 2]. The data shows the purified DNA is free of PCR inhibitors and the specific cfDNA concentration is higher than Company Q.

Circulating, cell-free DNA as small as 50 bp can be recovered with Mag-Bind® cfDNA Kit

Figure 3. DNA fragments as small as 50 bp can be recovered using modified protocol. Serum spiked with 50 bp DNA ladder was purified with the Mag-Bind® cfDNA Kit. By using modified protocols, smaller fragments were recovered.

Analysis of cfDNA purified from 8 mL plasma using Mag-Bind® cfDNA Kit

Figure 4.1. Plasma samples were purified using the Mag-Bind® cfDNA Kit. The purified DNA samples were loaded on a D100 high-sensitive tape on TapeStation® 2200.

Figure 4.2. Gel image from figure 4.1 was analyzed using the TapeStation software. Peak and concentration data showed that 8 mL automation process was a success.

Figure 4.3.  Gel image from figure 4.1 was analyzed using the TapeStation software. The overlay showed that the purified DNA contained more cfDNA and minimal genomic DNA contamination.

Citations

View Citations
  • Durvasula, Kiranmai, et al. “Strategies for retrieving cfDNA of short fragment lengths using Mag-Bind® cfDNA kit (M3298) from Omega Bio-tek.”
  • Komanduri. “Next-generation sequencing of microbial cell-free DNA for rapid noninvasive diagnosis of infectious diseases in immunocompromised hosts [ version 1 ; peer review : awaiting.” (2019).
  • Rowlands, Vicky, et al. “Optimisation of Robust Singleplex and Multiplex Droplet Digital PCR Assays for High Confidence Mutation Detection in Circulating Tumour DNA.” Scientific Reports, vol. 9, no. 1, 2 Sept. 2019, 10.1038/s41598-019-49043-x.
  • Zhang, Li, et al. “Effects of Simulated Warming on Soil Ammonia-Oxidizing Bacteria and Archaea Communities in an Alpine Forest of Western Sichuan, China.” Acta Ecologica Sinica, vol. 37, no. 2, 1 Apr. 2017, pp. 85–90, www.sciencedirect.com/science/article/pii/S1872203216301585?casa_token=Uw1wTvzFSaAAAAAA:zqW3kZfqRtQbRqj2wTqgdHnLUhu-GdeABY6JWBiVNU6oMawZy84GB4cRswdr9RRRlXGp4vvBSXo, 10.1016/j.chnaes.2016.12.004. Accessed 1 June 2020.
品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等