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当前位置: 首页 > 产品中心 > Cloned_Genes > Omega Bio-Tek/Mag-Bind®血液&;组织DNA HDQ 96试剂盒/1x96/M6399-00
商品详细Omega Bio-Tek/Mag-Bind®血液&;组织DNA HDQ 96试剂盒/1x96/M6399-00
Omega Bio-Tek/Mag-Bind®血液&;组织DNA HDQ 96试剂盒/1x96/M6399-00
Omega Bio-Tek/Mag-Bind®血液&;组织DNA HDQ 96试剂盒/1x96/M6399-00
商品编号: M6399-00
品牌: omegabiotek
市场价: ¥4494.00
美元价: 2247.00
产地: 美国(厂家直采)
公司:
产品分类: 克隆基因
公司分类: Cloned_Genes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

The Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is designed for isolation of high-quality genomic DNA from blood samples, saliva, swabs, mouse tails, dried blood spots or cultured cells. Mag-Bind® Particles HDQ provides quick magnetic response time reducing overall processing time. This system combines the reversible nucleic acid-binding properties of Mag-Bind® paramagnetic particles with Omega Bio-tek’s tissue and blood DNA isolation systems. Utilizing paramagnetic particles provides high-quality DNA that is suitable for direct use in most downstream applications such as Next Generation Sequencing, qPCR, PCR, and microarrays. The Mag-Bind Blood & Tissue DNA HDQ 96 Kit can be scaled up to process 1 mL whole blood samples.

For larger volumes, the Mag-Bind® Blood DNA HV Kit can be used.
  • Safe – No organic solvents
  • Compatible with DNA Genotek’s preservation system
  • Compatible with Mawi iSWAB DNA preservation system
  • High quality – Pure DNA suitable for downstream applications such as Next Generation Sequencing, qPCR, PCR, and microarrays
Protocols are available for the following automated platforms:
  • Hamilton Microlab® STAR
  • Hamilton Microlab® NIMBUS
  • KingFisher™, BioSprint®, and MagMAX® 96

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Starting Amount100-250 μL blood samples, saliva, swabs, mouse tails, dried blood spots or 5 x 106 cultured cells.
Elution Volume50-200 µL
TechnologyMagnetic Beads
Processing ModeAutomated, Manual
Throughput8 - 384
NoteDownstream applications: NGS, qPCR, microarray

Kit Components

ItemAvailable Separately
Mag-Bind® Particles HDQCall for Pricing
AL BufferView Product
TL BufferView Product
HDQ Binding BufferView Product
VHB BufferView Product
SPM Wash BufferView Product
Proteinase K SolutionView Product
Elution BufferView Product

Protocol and Resources

Product Documentation & Literature

PROTOCOL

M6399 Mag-Bind® Blood & Tissue DNA HDQ 96 Kit

SDS

M6399 SDS

SALES SHEET

VIDEO PROTOCOL

APPLICATION NOTE

High Throughput, Automated DNA Extraction Solution from Whole Blood Samples Using Omega Bio-tek’s Reagents on Tecan Fluent® 780 Workstation

APPLICATION NOTE

DNA Extraction from Whole Blood on Hamilton’s Microlab® STAR™

APPLICATION NOTE

Comprehensive, High Throughput Workflow for Automated gDNA Isolation from iSWAB Oral Samples

APPLICATION NOTE

A Simple, User-Friendly, High Throughput DNA Extraction Workflow Using 2-D Barcoded Buccal Swabs on Hamilton’s Firefly NIMBUS® 96

APPLICATION NOTE

Fully automated DNA extraction solution from Omega Bio-tek using saliva stabilized in Biomatrica’s Salivagard® HT DNA collection tubes

Product Data

DNA yield from 200 μL whole blood extracted using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit on the ABI MagMAX 96 instrument.

Figure 1.  DNA yield from 200 µL whole blood extracted using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit on the ABI MagMAX 96 instrument. DNA yield was determined by PicoGreen® quantification.

DNA yield from buccal swab samples using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is higher than a leading competitor.

Figure 2.  Genomic DNA was extracted from buccal swab samples using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit and Company A’s genomic DNA extraction kit. 10% of DNA extracted from each sample was analyzed on a 0.8% agarose gel.

Purified genomic DNA using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is of high quality and free of inhibitors.

Figure 3.  Genomic DNA was extracted from 1,500 µL whole blood using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit. The elution volume was 500 µL. DNA was analyzed on Thermo Scientific’s NanoDrop® 2000c. 50 ng of DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors.

DNA purified using Mag-Bind Blood & Tissue DNA HDQ 96 Kit was around 60 kb

Figure 4.  The sizes of genomic DNA purified using Mag-Bind Blood & Tissue DNA HDQ Kit were determined by electrophoresis.  Sample 1 and 2 were gDNA from human blood.  L is 50 kb genomic ladder for Agilent TapeStation 2200.  The size of gDNA is around 60 kb.

Citations

View Citations
  • V. Kilaru, S. V. Iyer, L. M. Almi, J. S. Stevens, A. Lori, T. Jovanovic, T. D. Ely, B. Bradley, E. B. Binder, N. Koen, D. J. Stein, K. N. Conneely, A. P. Wingo, A. K. Smith, K. J. Ressler. Genome-wide gene-based analysis suggests an association between Neuroligin 1 (NLGN1) and post-traumatic stress disorder
  • Xi Wang, Kai Wang, Wei Zhang, Ming Qiang, Ying Luo. A bilaminated decellularized scaffold for islet transportation: structure, properties and functions in diabetic mice
  • Pavel Dimens. Population structure of a migratory small coastal shark, the blacknose shark Carcharhinus acronotus, across cryptic barriers to gene flow
  • Molly Norton Darr. Biological studies and evaluation of Scymnus coniferarum crotch, a predator of hemlock woolly adelgid from western North America
Size

FREE SAMPLE, 1 x 96 Preps, 4 x 96 Preps

Format

Magnetic beads

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等