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当前位置: 首页 > 产品中心 > CRISPR > Omega Bio-Tek/E.Z.N.A.® Tissue DNA Kit/free-sample/D3396-00S
商品详细Omega Bio-Tek/E.Z.N.A.® Tissue DNA Kit/free-sample/D3396-00S
Omega Bio-Tek/E.Z.N.A.® Tissue DNA Kit/free-sample/D3396-00S
Omega Bio-Tek/E.Z.N.A.® Tissue DNA Kit/free-sample/D3396-00S
商品编号: D3396-00S
品牌: omegabiotek
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: CRISPR基因敲除
公司分类: CRISPR
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

The E.Z.N.A.® Tissue DNA Kit offers a versatile and cost-effective method for the isolation of DNA from a wide variety of samples including fresh or frozen animal cultured cells and tissues, buccal swabs, whole blood, mouse tail snips, etc. The DNA purification process is simplified with HiBind® Mini Column technology into four quick “lyse-bind-wash-elute” steps and can be accomplished in less than 20 minutes post-lysis. This convenient spin-column format avoids time-consuming steps like alcohol precipitation, use of toxic compounds such as phenol and chloroform and allows for multiple samples to be processed in parallel. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion.

  • Rapid – DNA isolation in less than 20 minutes post-lysis
  • Versatile – Single kit for multiple sample types
  • Specialized buffer system – Optimized buffers for higher yields
  • Safe – No phenol/chloroform extractions
  • High-quality – DNA is suitable for a variety of downstream applications

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Downstream ApplicationsPCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion
Starting MaterialTissues, cultured cells, mouse tail snips, paraffin-embedded tissues, whole blood, body fluids, buccal swabs
Starting Amount30 mg, or 5 x 106 cultured cells
Elution Volume100-200 μL
DNA Binding TechnologySilica mini spin column
Throughput1-24
Processing Mode< 20="" min="">

Kit Components

ItemAvailable Separately
HiBind® DNA Mini ColumnsView Product
2 mL Collection TubesView Product
BL BufferView Product
TL BufferView Product
HBC BufferView Product
DNA Wash BufferView Product
Elution BufferView Product
Proteinase K SolutionView Product

Protocol and Resources

Product Documentation & Literature

PROTOCOL

D3396 E.Z.N.A.® Tissue DNA Kit

SDS

D3396 SDS

QUICK GUIDE

D3396 Quick Guide

SALES SHEET

Product Data

Performance Comparison of E.Z.N.A.® Tissue DNA Kit Against Leading Competitors

Figure 1.  Purified genomic DNA from 10 mg rat kidney tissue was isolated using kits from Company T, Company A, Company P, Company Q, and Omega Bio-tek’s E.Z.N.A.® Tissue DNA Kit following manufacturer’s recommended protocols. 3% of eluted DNA was analyzed on a 0.8% agarose gel. M:Lambda-Hind III.

Real-time PCR of Genomic DNA Isolation with E.Z.N.A.® Tissue DNA Kit

Figure 2.  Genomic DNA was isolated from 10 mg of rat kidney with Omega Bio-tek’s E.Z.N.A.® Tissue DNA Kit. Serial dilutions of recovered genomic DNA were used as templates for real-time PCR amplification of a 100 bp fragment of the GAPDH gene with SYBR® Green labeling. Each reaction was performed in triplicate. The fluorescence versus cycle number is plotted and the 5 curves correspond to the input DNA template amounts of 10, 2, 0.4, 0.08, and 0.0016 ng.

Genomic DNA Yield From Mouse Liver

Table 1.  Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples and eluted in 100 µL volume. DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop ™ 2000c system.

Intact, high-molecular weight DNA as analyzed on an Agarose Gel

Figure 3.  Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples and eluted in 100 µL volume. 5 µL eluate was analyzed on a 1% Agarose gel.

Real-time PCR analysis of extracted DNA

Figure 4.  Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples. 2 µL of Eluted DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors.

Citations

View Citations
  • Pérez, L. M., Fittipaldi, M., Adrados, B., Morató, J., & Codony, F. (2013). Error estimation in environmental DNA targets quantification due to PCR efficiencies differences between real samples and standards. Folia microbiologica, 58(6), 657-662.
  • Dai, J., Wan, S., Zhou, F., Myers, R. E., Guo, X., Li, B., … & Xing, J. (2012). Genetic polymorphism in a VEGF-independent angiogenesis gene ANGPT1 and overall survival of colorectal cancer patients after surgical resection. PLoS One, 7(4), e34758.
  • Li, L., Luo, Y., Gao, Z., Huang, J., Zheng, X., Nie, H., … & Yuan, J. (2015). Molecular characterisation and prevalence of a new genotype of Cyprinid herpesvirus 2 in mainland China. Canadian journal of microbiology, 61(6), 381-387.
  • Pérez, L. M., Codony, F., Ríos, K., Peñuela, G., Adrados, B., Fittipaldi, M., … & Morató, J. (2012). Searching Simkania negevensis in environmental waters. Folia microbiologica, 57(1), 11-14.
  • Silva, F., Hernández-Miranda, E., & Brante, A. (2015). New polymorphic microsatellite markers for the pelagic fish Normanichthys crockeri. Conservation Genetics Resources, 7(2), 493-495.
  • Huang, W., Xiong, J., Yang, Y., Liu, S. M., Yuan, B. F., & Feng, Y. Q. (2015). Determination of DNA adenine methylation in genomes of mammals and plants by liquid chromatography/mass spectrometry. Rsc Advances, 5(79), 64046-64054.
  • Xu, S., Lou, F., Wu, Y., Sun, D. Q., Zhang, J. B., Chen, W., … & Wu, W. J. (2016). Circulating tumor DNA identified by targeted sequencing in advanced-stage non-small cell lung cancer patients. Cancer letters, 370(2), 324-331.
  • Ribas, A., Saijuntha, W., Agatsuma, T., Prantlová, V., & Poonlaphdecha, S. (2016). Rodents as a source of Salmonella contamination in wet markets in Thailand. Vector-Borne and Zoonotic Diseases, 16(8), 537-540.
  • Takano, O. M., Voelker, G., Gustafsson, D. R., & Light, J. E. (2019). Molecular phylogeny and novel host associations of avian chewing lice (I nsecta: P hthiraptera) from S outh A frica. Systematic Entomology, 44(2), 289-304.
  • Yu, L., Hu, Z., Gao, C., Feng, B., Wang, L., Tian, X., … & Wang, H. (2017). deletion of HPV18 E6 and E7 genes using dual sgRNA-directed CRISPR/Cas9 inhibits growth of cervical cancer cells. Int. J. Clin. Exp. Med, 10(6), 9206-9213.
  • Silva, F., Hernández-Miranda, E., & Brante, A. (2015). Development and characterization of microsatellite markers for the toadfish Aphos porosus. Conservation Genetics Resources, 7(2), 411-413.
  • Qu, F., Liu, X., Zhou, F., Yang, H., Bao, G., He, X., & Xing, J. (2011). Association between mitochondrial DNA content in leukocytes and colorectal cancer risk: a case‐control analysis. Cancer, 117(14), 3148-3155.
  • Saijuntha, W., Khumkratok, S., Wongpakam, K., Thanonkeo, S., Senakhun, C., Appamaraka, S., … & Tawong, W. (2017). Genetic diversity and population structure of blue-crested lizard, Calotes mystaceus Duméril & Bibron, 1837 (Squamata: Agamidae) in Thailand. Journal of genetics, 96(2), 377-382.
  • Wang, C., Zhou, Y., Lv, D., Ge, Y., Li, H., & You, Y. (2019). Change in the intestinal bacterial community structure associated with environmental microorganisms during the growth of Eriocheir sinensis. MicrobiologyOpen, 8(5), e00727.
  • de Dios, G., & Morató, J. (2012). Leonardo Martín Pérez, Francesc Codony, Karina Ríos, Gustavo Peñuela, Bárbara Adrados, Mariana Fittipaldi. Folia Microbiol, 57, 11-14. Alò, D., Correa, C., & Cárdenas, L. (2012). A1. 1 Abstract. Ecological impacts of invasive trout in Patagonian lakes, 155.
  • Dalton, C. S., van de Rakt, K., Fahlman, Å., Ruckstuhl, K., Neuhaus, P., Popko, R., … & van der Meer, F. (2017). Discovery of herpesviruses in Canadian wildlife. Archives of virology, 162(2), 449-456.
  • Warwick, A. R., & Lemmon, E. M. (2014). Development and characterization of 21 microsatellite loci for the Pine Barrens treefrog (Hyla andersonii). Conservation genetics resources, 6(3), 719-721.
  • Takano, O. M. (2016). Host Associations, Phylogenetics, and Biogeography of Parasitic Avian Chewing Lice (Insecta: Phthiraptera) from Sub-Saharan Africa (Doctoral dissertation).
  • Kumar, H. K. S., Gan, H. M., Tan, M. H., Eng, W. W. H., Barton, H. A., Hudson, A. O., & Savka, M. A. (2017). Genomic characterization of eight Ensifer strains isolated from pristine caves and a whole genome phylogeny of Ensifer (Sinorhizobium). Journal of genomics, 5, 12.
  • Sætre, C. L. C., Johnsen, A., Stensrud, E., & Cramer, E. R. (2018). Sperm morphology, sperm motility and paternity success in the bluethroat (Luscinia svecica). PloS one, 13(3), e0192644.
  • Zhao, X., Gu, K., Zeng, Q., Gao, L., & Cheng, D. (2019). Diagnostic Value of SjR2 Gene in Colonic Tissue from Schistosoma Japonicum Infected Hosts. Medical science monitor: international medical journal of experimental and clinical research, 25, 427.
  • Guo, N., Lou, F., Ma, Y., Li, J., Yang, B., Chen, W., … & Shi, R. (2016). Circulating tumor DNA detection in lung cancer patients before and after surgery. Scientific reports, 6, 33519.
  • Fittipaldi, M., Codony, F., Adrados, B., Camper, A. K., & Morató, J. (2011). Viable real-time PCR in environmental samples: can all data be interpreted directly?. Microbial ecology, 61(1), 7-12.
  • Xincheng, Z., Kunci, C., Xinping, Z., Jian, Z., Qing, L., Xiaoyou, H., … & Fengfang, X. (2015). Two molecular markers based on mitochondrial genomes for varieties identification of the northern snakehead (Channa argus) and blotched snakehead (Channa maculata) and their reciprocal hybrids. Mitochondrial DNA, 26(4), 555-558.
  • Chen, K. Z., Lou, F., Yang, F., Zhang, J. B., Ye, H., Chen, W., … & Jones, L. (2016). Circulating tumor DNA detection in early-stage non-small cell lung cancer patients by targeted sequencing. Scientific reports, 6, 31985.
  • JENNINGS-GAINES, J. E., EDWARDS, W. H., WOOD, M. E., FOX, K. A., WOLFE, L. L., MILLER, M. W., & KILLION, H. J. An Improved Method for Culturing Mycoplasma ovipneumoniae from Field Samples.
Format

Miniprep

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等