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当前位置: 首页 > 产品中心 > shelter > Omega Bio-Tek/Mag-Bind®病毒DNA/RNA 96试剂盒/12x96/M6246-03
商品详细Omega Bio-Tek/Mag-Bind®病毒DNA/RNA 96试剂盒/12x96/M6246-03
Omega Bio-Tek/Mag-Bind®病毒DNA/RNA 96试剂盒/12x96/M6246-03
Omega Bio-Tek/Mag-Bind®病毒DNA/RNA 96试剂盒/12x96/M6246-03
商品编号: M6246-03
品牌: omegabiotek
市场价: ¥43864.00
美元价: 21932.00
产地: 美国(厂家直采)
公司:
产品分类: 防护
公司分类: shelter
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Supplementary Protocol for NP swabs, aspirates and BAL samples

Overview

Mag-Bind® Viral DNA/RNA Kit is designed for the rapid and reliable isolation of viral RNA and viral DNA from serum, swabs, plasma, saliva, and other body fluids. The Mag-Bind® magnetic beads technology enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. In addition to easily being adapted with automated systems, this procedure can also be scaled up or down depending on the amount of starting sample. The purified nucleic acids are ready for direct use in downstream applications such as amplification or other enzymatic reactions.

Protocols are available for the following automated platforms:

  • Hamilton Microlab® STAR
  • Hamilton Microlab® NIMBUS
  • KingFisher™, BioSprint®, and MagMAX® 96
  • Adaptable to  other liquid handling platforms

Coronavirus (SARS-CoV-2) Extraction

Omega Bio-tek is assisting scientists, researchers and healthcare workers around the globe in accelerating the screening and detection of the novel coronavirus disease, COVID-19. We are supporting several Laboratory Developed Tests (LDTs) by providing high-throughput, automated viral nucleic acid extraction methodologies. To meet the exceptional and immediate need for supplies, Omega Bio-tek has ramped up the production of its Mag-Bind® Viral DNA/RNA 96 kit and has the capacity to support 1 million patient tests per month.

  • Supplement Protocol for NP swabs, aspirates and BAL samples
  • Fully automatable and ready-to-load scripts for: KingFisher™:  Email product_support@omegabiotek.com for script Hamilton Microlab STAR:  384 samples in ~3 hr Hamilton MagEx STARlet:   96 samples in ~1 hr 45 min
  • Dedicated technical and application support to expedite setup and validation time.
Contact a specialist

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Starting Amount50 µL - 200 µL
Starting MaterialSerum, plasma, saliva and other body fluids
Elution Volume20-50 μL
TechnologyMagnetic Beads
Processing ModeAutomated, Manual
Throughput8 - 96

Kit Components

ItemAvailable Separately
Mag-Bind® Particles CNRCall for Pricing
TNA Lysis Buffer---
VHB BufferView Product
Carrier RNA---
Proteinase K Solution (40 mg/mL)Call for Pricing
SPR Wash Buffer---
Nuclease-free WaterView Product

Protocol and Resources

Product Documentation & Literature

PROTOCOL

M6246 Mag-Bind® Viral DNA/RNA 96 Kit

PROTOCOL

M6246 Supplementary Protocol for NP Swabs, Aspirates and BAL Samples

SDS

M6246 SDS

SALES SHEET

Product Data

Mag-Bind ® Viral DNA/RNA Kit had better yield than a leading competing product

Figure 1. HBV virus (in quantities of 10 and 1 infectious unit[s]) was spiked into 200 µL human serum. Viral nucleic acid was isolated with Omega Bio-tek’s Mag-Bind® Viral DNA/RNA Kit and with a comparable kit from Company A according to recommended protocols. 5 µL of template was used for a SYBR® Green-labeled qPCR reaction which was replicated 4 times. The resulting mean Ct values are shown in the above figure.

DNA/RNA purified with Mag-Bind® Viral DNA/RNA had less inhibitor than with a leading competing product

Figure 2. Nucleic acid was isolated from 200 µL of human whole blood with Omega Bio-tek’s Mag-Bind® Viral DNA/RNA Kit and a comparable kit from Company A using the manufacturer’s recommended protocols. The extractions were eluted in 100 µL. Three concentrations of template were used as templates in a SYBR® Green-labeled qPCR reaction. Each reaction was performed in quadruplicate and the mean Ct value is depicted in the above figure.

Figure 3. 50 µL of ZeptoMetrix’s NATtrol Influenza A/B Positive Control standard was spiked into 150 µL of human serum and viral nucleic acids were extracted using Omega Bio-tek’s Mag-Bind® Viral DNA/RNA 96 Kit. Average Ct values obtained after amplification using Influenza B primers are shown above. The results indicate positive detection of Influenza B in both undiluted and 10-fold diluted purified samples.

Table 1.  Some of the viruses* detected using our viral kits.

Influenza AHepatitis ESheep pox virus
Influenza BInfectious Bronchitis virusMurine norovirus 1
West Nile virusPorcine reproductive and respiratory syndrome Virus (PRRSV)Canine distemper virus
Middle East Respiratory Syndrome Coronavirus (MERS-CoV)Insect-specific flaviviruses, mononegaviruses, and totivirusesRabies virus
Zika virus (ZIKAV)orf virus (ORFV)Rotavirus
SIVPorcine circovirus type 2 (PCV2)Coxsackievirus B3
HIVArbovirusesCoxsackievirus A6
Influenza A (H1N1)Dengue virusAvian leukosis virus subgroup J
Hepatitis A virus types 1 and 3GB virus CAvian Encephalomyelitis Virus
Hepatitis B virusBovine Viral Diarrhea Virus (BVDV)Crimean-Congo hemorrhagic fever virus
*References available upon request

Publications

  • Fauver, Joseph R., Shamima Akter, et al. “A Reverse-Transcription/RNase H Based Protocol for Depletion of Mosquito Ribosomal RNA Facilitates Viral Intrahost Evolution Analysis, Transcriptomics and Pathogen Discovery.” Virology, vol. 528, Feb. 2019, pp. 181–197, doi:10.1016/j.virol.2018.12.020.‌
  • Gu, Shao-peng, et al. “Identification and Phylogenetic Analysis of the Sheep Pox Virus Shanxi Isolate.” Www.Biomedres.Info, 2018, www.biomedres.info/biomedical-research/identification-and-phylogenetic-analysis-of-the-sheep-pox-virus-shanxi-isolate-9658.html.‌
  • Fauver, Joseph R., James Weger-Lucarelli, et al. “Xenosurveillance Reflects Traditional Sampling Techniques for the Identification of Human Pathogens: A Comparative Study in West Africa.” PLOS Neglected Tropical Diseases, edited by Paulo Filemon Pimenta, vol. 12, no. 3, Mar. 2018, p. e0006348, doi:10.1371/journal.pntd.0006348.‌
  • Prakash, Ravi, et al. “Detection of Arboviruses in Blood and Mosquito Slurry Samples Using Polymer Microchip.” IEEE Xplore, 1 Nov. 2017, pp. 168–171, doi:10.1109/HIC.2017.8227611.‌
  • Gloria-Soria, A., et al. “Infection Rate of Aedes Aegyptimosquitoes with Dengue Virus Depends on the Interaction between Temperature and Mosquito Genotype.” Proceedings of the Royal Society B: Biological Sciences, vol. 284, no. 1864, Oct. 2017, p. 20171506, doi:10.1098/rspb.2017.1506.
View More Publications
  • Spivey, Timothy J., et al. “Maintenance of Influenza A Viruses and Antibody Response in Mallards (Anas Platyrhynchos) Sampled during the Non-Breeding Season in Alaska.” PLOS ONE, edited by Balaji Manicassamy, vol. 12, no. 8, Aug. 2017, p. e0183505, doi:10.1371/journal.pone.0183505.‌
  • Fauver, Joseph R., Brian D. Foy, et al. “The Use of Xenosurveillance to Detect Human Bacteria, Parasites, and Viruses in Mosquito Bloodmeals.” The American Journal of Tropical Medicine and Hygiene, vol. 97, no. 2, Aug. 2017, pp. 324–29, doi:10.4269/ajtmh.17-0063.‌
  • Yu, Yongzhong, et al. “Characterization of an Orf Virus Isolate from an Outbreak in Heilongjiang Province, China.” Archives of Virology, vol. 162, no. 10, June 2017, pp. 3143–49, doi:10.1007/s00705-017-3426-x.‌
  • Rückert, Claudia, et al. “Impact of Simultaneous Exposure to Arboviruses on Infection and Transmission by Aedes Aegypti Mosquitoes.” Nature Communications, vol. 8, May 2017, doi:10.1038/ncomms15412.
  • Chotiwan, Nunya, et al. “Rapid and Specific Detection of Asian- and African-Lineage Zika Viruses.” Science Translational Medicine, vol. 9, no. 388, May 2017, doi:10.1126/scitranslmed.aag0538.‌
  • Law, Jessica, et al. “Induction of Humoral Immune Response in Piglets after Perinatal or Post-Weaning Immunization against Porcine Circovirus Type-2 or Keyhole Limpet Hemocyanin.” Canadian Journal of Veterinary Research, vol. 81, no. 1, Jan. 2017, pp. 5–11, www.ncbi.nlm.nih.gov/pmc/articles/PMC5220598/.‌
  • Grubaugh, Nathan D., et al. “Transmission Bottlenecks and RNAi Collectively Influence Tick-Borne Flavivirus Evolution.” Virus Evolution, vol. 2, no. 2, July 2016, doi:10.1093/ve/vew033.
  • Puryear, Wendy Blay, et al. “Prevalence of Influenza A Virus in Live-Captured North Atlantic Gray Seals: A Possible Wild Reservoir.” Emerging Microbes & Infections, vol. 5, no. 1, Jan. 2016, pp. 1–9, doi:10.1038/emi.2016.77.
  • Bliss, N., et al. “Prevalence of Influenza A Virus in Exhibition Swine during Arrival at Agricultural Fairs.” Zoonoses and Public Health, vol. 63, no. 6, Jan. 2016, pp. 477–85, doi:10.1111/zph.12252.‌
  • McCorkell, Robert, et al. “Acute BVDV-2 Infection in Beef Calves Delays Humoral Responses to a Non-Infectious Antigen Challenge.” The Canadian Veterinary Journal, vol. 56, no. 10, Oct. 2015, pp. 1075–1083, www.ncbi.nlm.nih.gov/pmc/articles/PMC4572827/.‌
  • Solis Worsfold, Cristina, et al. “Assessment of Neutralizing and Non-Neutralizing Antibody Responses against Porcine Circovirus 2 in Vaccinated and Non-Vaccinated Farmed Pigs.” Journal of General Virology, vol. 96, no. 9, Sept. 2015, pp. 2743–48, doi:10.1099/vir.0.000206.
  • Eschbaumer, Michael, et al. “Probe-Free Real-Time Reverse Transcription Polymerase Chain Reaction Assays for the Detection and Typing of Porcine Reproductive and Respiratory Syndrome Virus in Canada.” Canadian Journal of Veterinary Research, vol. 79, no. 3, July 2015, pp. 170–179, www.ncbi.nlm.nih.gov/pmc/articles/PMC4445508/.
  • Dow, Natalie, et al. “Genetic Variability of Bovine Viral Diarrhea Virus and Evidence for a Possible Genetic Bottleneck during Vertical Transmission in Persistently Infected Cattle.” PLOS ONE, edited by Binu T Velayudhan, vol. 10, no. 7, July 2015, p. e0131972, doi:10.1371/journal.pone.0131972.
  • ‌Yan, Mengfei, et al. “Infection of Porcine Circovirus 2 (PCV2) in Intestinal Porcine Epithelial Cell Line (IPEC-J2) and Interaction between PCV2 and IPEC-J2 Microfilaments.” Virology Journal, vol. 11, no. 1, Nov. 2014, doi:10.1186/s12985-014-0193-0.
Format

Magnetic beads

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等