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当前位置: 首页 > 产品中心 > Transfection > Omega Bio-Tek/E.Z.N.A.?循环DNA试剂盒/免费样品/D3091-00S
商品详细Omega Bio-Tek/E.Z.N.A.?循环DNA试剂盒/免费样品/D3091-00S
Omega Bio-Tek/E.Z.N.A.?循环DNA试剂盒/免费样品/D3091-00S
Omega Bio-Tek/E.Z.N.A.?循环DNA试剂盒/免费样品/D3091-00S
商品编号: D3091-00S
品牌: omegabiotek
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 转染试剂
公司分类: Transfection
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview

The E.Z.N.A.® Circulating DNA Kit provides a rapid and easy method for the isolation of circulating DNA from plasma, serum, and other acellular body fluids. The kit allows for simultaneous processing of single or multiple samples in under 120 minutes. DNA purified using the E.Z.N.A.® Circulating DNA method is ready for applications such as PCR, microarrays, and next generation sequencing.

E.Z.N.A.® Circulating DNA Kit uses the reversible nucleic acid binding properties of our HiBind® matrix combined with the speed of mini column centrifugation and funnel adaptors. The funnel adaptors allow for large volumes of liquid to be applied to the mini columns when using a vacuum manifold.

A specially formulated buffer system allows circulating DNA to bind to the HiBind® matrix. Samples are lysed under denaturing conditions and then transferred to the DNA column where DNA binds and cellular debris, hemoglobin, and other proteins are washed away. High-quality DNA is eluted in nuclease-free water.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FeaturesSpecifications
Starting Amount1-4 mL cell-free fluid
Starting MaterialPlasma, serum, and other acellular body fluids
Elution Volume30-150 μL
TechnologyHiBind® DNA Mini Column
Processing ModeManual
Throughput1-24

Kit Components

ItemAvailable Separately
Circulating DNA Mini Columns---
#6 Column Funnel---
2 mL Collection TubesView Product
DS BufferCall for Pricing
JSB BufferView Product
VHB BufferView Product
SPW Wash BufferView Product
Elution BufferView Product
Proteinase K SolutionView Product

Protocol and Resources

Product Documentation & Literature

PROTOCOL

D3091 E.Z.N.A.® Circulating DNA Kit

SDS

D3091 SDS

SALES SHEET

APPLICATION NOTE

Extraction & Quality Analysis of Circulating, Cell-Free DNA from Serum Samples Using Omega Bio-tek Kits

Product Data

Circulating cfDNA purified by E.Z.N.A.® Circulating DNA Kit has excellent yield and minimal genomic DNA contamination.

Figure 1.  Electropherogram overlay of purified DNA from 4 mL serum. 4 mL of unspiked serum was purified using kits from Omega Bio-tek and Company Q following manufacturer’s recommended protocols. Purified DNA was analyzed on Agilent’s TapeStation® 2200.

E.Z.N.A.® Circulating DNA Kit uses less volumes of lysis/binding buffers and the whole process takes less time than the leading competitor.

Figure 2. 

Citations

View Citations
  • Zhao, Xu, et al. “Diagnostic Value of SjR2 Gene in Colonic Tissue from Schistosoma Japonicum Infected Hosts.” Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 25, 14 Jan. 2019, pp. 427–435, www.ncbi.nlm.nih.gov/pmc/articles/PMC6342060/, 10.12659/MSM.912997.

Size

FREE SAMPLE, 5 preps, 50 preps

Format

Miniprep

品牌介绍
Omega Bio-Tek公司自1998年成立以来,核酸纯化技术一直处于前列.Omega 第二代Hibind 硅胶柱具有很大的灵活性,利用硅胶膜的优势,可以从动物,植物,培养细胞,凝胶和溶液中提取和纯化DNA/RNA, 产品覆盖整个核酸领域,并且稳定的质量和优质的服务一直受到全球的科研,企业客户所喜爱.2004年,Omega Bio-tek公司授权广州飞扬生物工程有限公司为中国总代理,为国内科研和企业客户进行服务.Omega Bio-Tek公司的主要客户:孟山都、美国农业部等